Detailed information of TA system TA159764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 493152..493789
• Replicon: chromosome
• Accession: NZ_CP054467
• Organism: Bacillus velezensis strain CF57

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HSX12_RS02525
• Protein ID: WP_003156187.1
• Coordinates: 493439..493789 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HSX12_RS02520
• Protein ID: WP_003156188.1
• Coordinates: 493152..493433 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HSX12_RS02500 (HSX12_02470)	489517..490116	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
HSX12_RS02505 (HSX12_02475)	490209..490574	+	366	WP_007410229.1	holo-ACP synthase	-
HSX12_RS02510 (HSX12_02480)	490739..491746	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
HSX12_RS02515 (HSX12_02485)	491863..493032	+	1170	WP_061890435.1	alanine racemase	-
HSX12_RS02520 (HSX12_02490)	493152..493433	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HSX12_RS02525 (HSX12_02495)	493439..493789	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HSX12_RS02530 (HSX12_02500)	493907..494728	+	822	WP_015416874.1	STAS domain-containing protein	-
HSX12_RS02535 (HSX12_02505)	494733..495098	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HSX12_RS02540 (HSX12_02510)	495101..495502	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HSX12_RS02545 (HSX12_02515)	495514..496521	+	1008	WP_015416875.1	PP2C family protein-serine/threonine phosphatase	-
HSX12_RS02550 (HSX12_02520)	496585..496914	+	330	WP_033575044.1	anti-sigma factor antagonist	-
HSX12_RS02555 (HSX12_02525)	496911..497393	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
HSX12_RS02560 (HSX12_02530)	497359..498147	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
HSX12_RS02565 (HSX12_02535)	498147..498749	+	603	WP_007410234.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T159764 WP_003156187.1 NZ_CP054467:493439-493789 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T159764 NZ_CP070134:c830033-829779 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT159764 WP_003156188.1 NZ_CP054467:493152-493433 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT159764 NZ_CP070134:c830281-830030 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5