159729

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1393919..1394835 Replicon chromosome
Accession NZ_CP054415
Organism Bacillus amyloliquefaciens strain 205

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A7U5AVM4
Locus tag HTY60_RS07530 Protein ID WP_013351967.1
Coordinates 1394089..1394835 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag HTY60_RS07525 Protein ID WP_003154807.1
Coordinates 1393919..1394089 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTY60_RS07485 1389198..1389827 + 630 Protein_1415 hypothetical protein -
HTY60_RS07490 1390311..1390772 + 462 WP_127721186.1 hypothetical protein -
HTY60_RS07495 1390785..1391183 + 399 WP_174201931.1 hypothetical protein -
HTY60_RS07500 1391170..1391367 + 198 WP_013351962.1 XkdX family protein -
HTY60_RS07505 1391416..1392177 + 762 WP_013351963.1 hypothetical protein -
HTY60_RS07510 1392231..1392494 + 264 WP_013351964.1 hemolysin XhlA family protein -
HTY60_RS07515 1392508..1392771 + 264 WP_013351965.1 phage holin -
HTY60_RS07520 1392785..1393663 + 879 WP_013351966.1 N-acetylmuramoyl-L-alanine amidase -
HTY60_RS07525 1393919..1394089 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
HTY60_RS07530 1394089..1394835 - 747 WP_013351967.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
HTY60_RS07535 1394943..1395941 - 999 WP_013351968.1 inorganic phosphate transporter -
HTY60_RS07540 1395954..1396571 - 618 WP_003154804.1 DUF47 domain-containing protein -
HTY60_RS07545 1396856..1398172 - 1317 WP_013351969.1 amino acid permease -
HTY60_RS07550 1398495..1399445 + 951 WP_013351970.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29054.53 Da        Isoelectric Point: 4.6839

>T159729 WP_013351967.1 NZ_CP054415:c1394835-1394089 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T159729 NZ_CP070115:4518042-4518149 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT159729 WP_003154807.1 NZ_CP054415:c1394089-1393919 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT159729 NZ_CP070115:c4517989-4517928 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U5AVM4

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References