159728

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 474452..475089 Replicon chromosome
Accession NZ_CP054415
Organism Bacillus amyloliquefaciens strain 205

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HTY60_RS02405 Protein ID WP_003156187.1
Coordinates 474739..475089 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HTY60_RS02400 Protein ID WP_003156188.1
Coordinates 474452..474733 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTY60_RS02380 470818..471417 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
HTY60_RS02385 471510..471875 + 366 WP_013351093.1 holo-ACP synthase -
HTY60_RS02390 472040..473047 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
HTY60_RS02395 473164..474333 + 1170 WP_013351095.1 alanine racemase -
HTY60_RS02400 474452..474733 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HTY60_RS02405 474739..475089 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HTY60_RS02410 475210..476031 + 822 WP_174201891.1 STAS domain-containing protein -
HTY60_RS02415 476036..476401 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HTY60_RS02420 476404..476805 + 402 WP_013351097.1 anti-sigma regulatory factor -
HTY60_RS02425 476817..477824 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
HTY60_RS02430 477888..478217 + 330 WP_013351099.1 anti-sigma factor antagonist -
HTY60_RS02435 478214..478696 + 483 WP_174201892.1 anti-sigma B factor RsbW -
HTY60_RS02440 478662..479450 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
HTY60_RS02445 479450..480052 + 603 WP_013351102.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T159728 WP_003156187.1 NZ_CP054415:474739-475089 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T159728 NZ_CP070115:4243250-4243528 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT159728 WP_003156188.1 NZ_CP054415:474452-474733 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT159728 NZ_CP070115:4243528-4243812 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References