Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76023..76666 Replicon plasmid pSCU-204-1
Accession NZ_CP054414
Organism Escherichia coli strain SCU-204

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HHJ42_RS26055 Protein ID WP_001034046.1
Coordinates 76250..76666 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HHJ42_RS26050 Protein ID WP_001261278.1
Coordinates 76023..76253 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ42_RS26020 (HHJ42_025135) 71534..71839 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HHJ42_RS26025 (HHJ42_025140) 71841..72059 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HHJ42_RS26030 (HHJ42_025145) 72626..73138 + 513 WP_000151784.1 hypothetical protein -
HHJ42_RS26035 (HHJ42_025150) 73172..74305 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HHJ42_RS26040 (HHJ42_025155) 74472..75245 - 774 WP_000905949.1 hypothetical protein -
HHJ42_RS26045 (HHJ42_025160) 75258..75758 - 501 WP_001773886.1 HEPN family nuclease -
HHJ42_RS26050 (HHJ42_025165) 76023..76253 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HHJ42_RS26055 (HHJ42_025170) 76250..76666 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HHJ42_RS26060 (HHJ42_025175) 76711..80505 - 3795 WP_001144732.1 hypothetical protein -
HHJ42_RS26065 (HHJ42_025180) 80886..81116 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
HHJ42_RS26070 (HHJ42_025185) 81113..81529 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id senB 1..88052 88052


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T159726 WP_001034046.1 NZ_CP054414:76250-76666 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T159726 NZ_CP070115:3920599-3920701 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT159726 WP_001261278.1 NZ_CP054414:76023-76253 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT159726 NZ_CP070115:c3920739-3920471 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References