Detailed information of TA system TA159725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 71534..72059
• Replicon: plasmid pSCU-204-1
• Accession: NZ_CP054414
• Organism: Escherichia coli strain SCU-204

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: HHJ42_RS26020
• Protein ID: WP_001159868.1
• Coordinates: 71534..71839 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: HHJ42_RS26025
• Protein ID: WP_000813634.1
• Coordinates: 71841..72059 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ42_RS26005 (HHJ42_025120)	67444..68610	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
HHJ42_RS26010 (HHJ42_025125)	69198..69953	-	756	WP_000852146.1	replication initiation protein RepE	-
HHJ42_RS26015 (HHJ42_025130)	70727..71533	-	807	WP_000016982.1	site-specific integrase	-
HHJ42_RS26020 (HHJ42_025135)	71534..71839	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
HHJ42_RS26025 (HHJ42_025140)	71841..72059	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HHJ42_RS26030 (HHJ42_025145)	72626..73138	+	513	WP_000151784.1	hypothetical protein	-
HHJ42_RS26035 (HHJ42_025150)	73172..74305	-	1134	WP_001542067.1	DUF3800 domain-containing protein	-
HHJ42_RS26040 (HHJ42_025155)	74472..75245	-	774	WP_000905949.1	hypothetical protein	-
HHJ42_RS26045 (HHJ42_025160)	75258..75758	-	501	WP_001773886.1	HEPN family nuclease	-
HHJ42_RS26050 (HHJ42_025165)	76023..76253	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
HHJ42_RS26055 (HHJ42_025170)	76250..76666	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	senB	1..88052	88052

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T159725 WP_001159868.1 NZ_CP054414:c71839-71534 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T159725 NZ_CP070115:c3653230-3652856 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT159725 WP_000813634.1 NZ_CP054414:c72059-71841 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT159725 NZ_CP070115:c3653687-3653319 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2