Detailed information of TA system TA159718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3091723..3093506
• Replicon: chromosome
• Accession: NZ_CP054411
• Organism: Enterobacter hormaechei strain KCJ3K19

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: FCG87_RS14770
• Protein ID: WP_045331150.1
• Coordinates: 3091723..3093072 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: FCG87_RS14775
• Protein ID: WP_003859317.1
• Coordinates: 3093069..3093506 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCG87_RS14745 (FCG87_014750)	3087546..3088481	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
FCG87_RS14750 (FCG87_014755)	3088650..3089045	+	396	WP_003859308.1	CidA/LrgA family protein	-
FCG87_RS14755 (FCG87_014760)	3089042..3089737	+	696	WP_003859310.1	CidB/LrgB family autolysis modulator	-
FCG87_RS14760 (FCG87_014765)	3089866..3090750	+	885	WP_003859312.1	cytidine deaminase	-
FCG87_RS14765 (FCG87_014770)	3090878..3091588	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
FCG87_RS14770 (FCG87_014775)	3091723..3093072	+	1350	WP_045331150.1	HipA domain-containing protein	Toxin
FCG87_RS14775 (FCG87_014780)	3093069..3093506	+	438	WP_003859317.1	helix-turn-helix transcriptional regulator	Antitoxin
FCG87_RS14780 (FCG87_014785)	3093545..3094555	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
FCG87_RS14785 (FCG87_014790)	3094571..3096091	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
FCG87_RS14790 (FCG87_014795)	3096171..3097169	-	999	WP_045337654.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
FCG87_RS14795 (FCG87_014800)	3097465..3098487	-	1023	WP_003859324.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51140.83 Da
• Isoelectric Point: 4.6112

>T159718 WP_045331150.1 NZ_CP054411:3091723-3093072 [Enterobacter hormaechei]
METLTVQAYLNETWTDIALIKYPGSEKNDWNITQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYQFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T159718 NZ_CP070115:1786600-1786707 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT159718 WP_003859317.1 NZ_CP054411:3093069-3093506 [Enterobacter hormaechei]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT159718 NZ_CP070115:c1786543-1786486 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A837FFV9