Detailed information of TA system TA159709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2353956..2354695
• Replicon: chromosome
• Accession: NZ_CP054411
• Organism: Enterobacter hormaechei strain KCJ3K19

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: FCG87_RS11300
• Protein ID: WP_003857133.1
• Coordinates: 2353956..2354441 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A927HKN6
• Locus tag: FCG87_RS11305
• Protein ID: WP_045336276.1
• Coordinates: 2354429..2354695 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCG87_RS11270 (FCG87_011280)	2349447..2350019	+	573	WP_045346197.1	NAD(P)H-dependent oxidoreductase	-
FCG87_RS11275 (FCG87_011285)	2350022..2351041	+	1020	WP_045346198.1	aldo/keto reductase	-
FCG87_RS11280 (FCG87_011290)	2351080..2351457	+	378	WP_023292403.1	DUF1330 domain-containing protein	-
FCG87_RS11285 (FCG87_011295)	2351551..2352099	+	549	WP_045346251.1	NAD(P)H-dependent oxidoreductase	-
FCG87_RS11290 (FCG87_011300)	2352140..2352835	+	696	WP_170970030.1	antibiotic biosynthesis monooxygenase	-
FCG87_RS11295 (FCG87_011305)	2352851..2353654	+	804	WP_045346200.1	carboxymuconolactone decarboxylase family protein	-
FCG87_RS11300 (FCG87_011310)	2353956..2354441	-	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
FCG87_RS11305 (FCG87_011315)	2354429..2354695	-	267	WP_045336276.1	DUF1778 domain-containing protein	Antitoxin
FCG87_RS11310 (FCG87_011320)	2354759..2355688	-	930	WP_060579891.1	LysR family transcriptional regulator	-
FCG87_RS11315 (FCG87_011325)	2355818..2357206	+	1389	WP_058658946.1	MFS transporter	-
FCG87_RS11320 (FCG87_011330)	2357228..2358223	-	996	WP_032658432.1	DUF2891 domain-containing protein	-
FCG87_RS11325 (FCG87_011335)	2358233..2359219	-	987	WP_003857123.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2339592..2354695	15103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T159709 WP_003857133.1 NZ_CP054411:c2354441-2353956 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T159709 NZ_CP070115:c183133-182831 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.46 Da
• Isoelectric Point: 4.4857

>AT159709 WP_045336276.1 NZ_CP054411:c2354695-2354429 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWGV

Nucleotide

• Length: 267 bp

>AT159709 NZ_CP070115:c182795-182454 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9