Detailed information of TA system TA159691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 298704..299300
• Replicon: chromosome
• Accession: NZ_CP054410
• Organism: Enterobacter hormaechei strain KCJ3K13

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: FCG85_RS01725
• Protein ID: WP_023315213.1
• Coordinates: 298998..299300 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FCG85_RS01720
• Protein ID: WP_032610152.1
• Coordinates: 298704..298991 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FCG85_RS01715 (FCG85_001715)	297076..298707	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
FCG85_RS01720 (FCG85_001720)	298704..298991	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
FCG85_RS01725 (FCG85_001725)	298998..299300	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FCG85_RS01730 (FCG85_001730)	299498..300370	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
FCG85_RS01735 (FCG85_001735)	300371..300643	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
FCG85_RS01740 (FCG85_001740)	300694..301638	-	945	WP_032608239.1	ketopantoate/pantoate/pantothenate transporter PanS	-
FCG85_RS01745 (FCG85_001745)	301732..303081	-	1350	WP_100154725.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T159691 WP_023315213.1 NZ_CP054410:c299300-298998 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T159691 NZ_CP070111:1049201-1049479 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT159691 WP_032610152.1 NZ_CP054410:c298991-298704 [Enterobacter hormaechei]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT159691 NZ_CP070111:1049479-1049763 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8