159684

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3640115..3640733 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FR957_RS18030 Protein ID WP_001291435.1
Coordinates 3640515..3640733 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FR957_RS18025 Protein ID WP_000344800.1
Coordinates 3640115..3640489 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS18015 (3635204) 3635204..3636397 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FR957_RS18020 (3636420) 3636420..3639569 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FR957_RS18025 (3640115) 3640115..3640489 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FR957_RS18030 (3640515) 3640515..3640733 + 219 WP_001291435.1 HHA domain-containing protein Toxin
FR957_RS18035 (3640904) 3640904..3641455 + 552 WP_000102556.1 maltose O-acetyltransferase -
FR957_RS18040 (3641571) 3641571..3642041 + 471 WP_000136192.1 YlaC family protein -
FR957_RS18045 (3642205) 3642205..3643755 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FR957_RS18050 (3643797) 3643797..3644150 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FR957_RS18060 (3644529) 3644529..3644840 + 312 WP_000409911.1 MGMT family protein -
FR957_RS18065 (3644871) 3644871..3645443 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159684 WP_001291435.1 NZ_CP054407:3640515-3640733 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T159684 NZ_CP070111:407504-407755 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT159684 WP_000344800.1 NZ_CP054407:3640115-3640489 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT159684 NZ_CP070111:407752-408006 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References