Detailed information of TA system TA159674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2468674..2469312
• Replicon: chromosome
• Accession: NZ_CP054407
• Organism: Escherichia coli strain KCJ3K291

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: FR957_RS12250
• Protein ID: WP_000813794.1
• Coordinates: 2468674..2468850 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: FR957_RS12255
• Protein ID: WP_001270286.1
• Coordinates: 2468896..2469312 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FR957_RS12230 (2464293)	2464293..2465468	-	1176	WP_001583501.1	BenE family transporter YdcO	-
FR957_RS12235 (2465560)	2465560..2466096	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
FR957_RS12240 (2466169)	2466169..2468130	+	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
FR957_RS12245 (2468222)	2468222..2468452	-	231	WP_000494244.1	YncJ family protein	-
FR957_RS12250 (2468674)	2468674..2468850	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
FR957_RS12255 (2468896)	2468896..2469312	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
FR957_RS12260 (2469391)	2469391..2470797	+	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
FR957_RS12265 (2471042)	2471042..2472187	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
FR957_RS12270 (2472205)	2472205..2473218	+	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
FR957_RS12275 (2473219)	2473219..2474160	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2463105..2464253	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T159674 WP_000813794.1 NZ_CP054407:2468674-2468850 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T159674 NZ_CP070105:c1285960-1285475 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT159674 WP_001270286.1 NZ_CP054407:2468896-2469312 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT159674 NZ_CP070105:c1286250-1285957 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• PDB: 6HPB