159666

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1441957..1442582 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag FR957_RS07070 Protein ID WP_000911331.1
Coordinates 1442184..1442582 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FR957_RS07065 Protein ID WP_000450524.1
Coordinates 1441957..1442184 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS07040 (1437760) 1437760..1438230 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FR957_RS07045 (1438230) 1438230..1438802 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FR957_RS07050 (1438948) 1438948..1439826 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FR957_RS07055 (1439843) 1439843..1440877 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
FR957_RS07060 (1441090) 1441090..1441803 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FR957_RS07065 (1441957) 1441957..1442184 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FR957_RS07070 (1442184) 1442184..1442582 + 399 WP_000911331.1 type II toxin-antitoxin system VapC family toxin Toxin
FR957_RS07075 (1442729) 1442729..1443592 + 864 WP_001267507.1 neutral zinc metallopeptidase -
FR957_RS07080 (1443607) 1443607..1445622 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
FR957_RS07085 (1445696) 1445696..1446394 + 699 WP_000679812.1 esterase -
FR957_RS07090 (1446504) 1446504..1446704 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - algU 1303319..1470757 167438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T159666 WP_000911331.1 NZ_CP054407:1442184-1442582 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T159666 NZ_CP070103:3653429-3653605 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT159666 WP_000450524.1 NZ_CP054407:1441957-1442184 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT159666 NZ_CP070103:3653651-3654067 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References