159665

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1019653..1020236 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag FR957_RS04960 Protein ID WP_000254745.1
Coordinates 1019901..1020236 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag FR957_RS04955 Protein ID WP_000581939.1
Coordinates 1019653..1019901 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS04945 (1015992) 1015992..1017293 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
FR957_RS04950 (1017341) 1017341..1019575 + 2235 WP_000226815.1 GTP pyrophosphokinase -
FR957_RS04955 (1019653) 1019653..1019901 + 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
FR957_RS04960 (1019901) 1019901..1020236 + 336 WP_000254745.1 endoribonuclease MazF Toxin
FR957_RS04965 (1020307) 1020307..1021098 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
FR957_RS04970 (1021326) 1021326..1022963 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
FR957_RS04975 (1023051) 1023051..1024349 + 1299 WP_000036723.1 phosphopyruvate hydratase -
FR957_RS04980 (1024409) 1024409..1024904 - 496 Protein_973 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1024918..1025898 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T159665 WP_000254745.1 NZ_CP054407:1019901-1020236 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T159665 NZ_CP070103:c3442359-3442252 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT159665 WP_000581939.1 NZ_CP054407:1019653-1019901 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT159665 NZ_CP070103:3442406-3442472 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References