159662

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 274561..275147 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag FR957_RS01230 Protein ID WP_042014162.1
Coordinates 274561..274791 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FR957_RS01235 Protein ID WP_000593555.1
Coordinates 274788..275147 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS01220 (270598) 270598..273333 + 2736 WP_000149158.1 ribosome-associated ATPase/putative transporter RbbA -
FR957_RS01225 (273333) 273333..274457 + 1125 WP_001314210.1 ABC transporter permease -
FR957_RS01230 (274561) 274561..274791 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
FR957_RS01235 (274788) 274788..275147 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FR957_RS01240 (275267) 275267..275668 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
FR957_RS01245 (275674) 275674..276480 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
FR957_RS01250 (276477) 276477..277241 - 765 WP_032218476.1 nickel import ATP-binding protein NikD -
FR957_RS01255 (277241) 277241..278074 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
FR957_RS01260 (278071) 278071..279015 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T159662 WP_042014162.1 NZ_CP054407:274561-274791 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T159662 NZ_CP070103:c2706428-2706210 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT159662 WP_000593555.1 NZ_CP054407:274788-275147 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT159662 NZ_CP070103:c2706828-2706454 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References