Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5047960..5048585 Replicon chromosome
Accession NZ_CP054406
Organism Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FOH49_RS26335 Protein ID WP_002882817.1
Coordinates 5047960..5048343 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FOH49_RS26340 Protein ID WP_004150355.1
Coordinates 5048343..5048585 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH49_RS26320 (FOH49_026365) 5045326..5046228 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FOH49_RS26325 (FOH49_026370) 5046225..5046860 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FOH49_RS26330 (FOH49_026375) 5046857..5047786 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FOH49_RS26335 (FOH49_026380) 5047960..5048343 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FOH49_RS26340 (FOH49_026385) 5048343..5048585 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FOH49_RS26345 (FOH49_026390) 5048790..5049707 + 918 WP_004181612.1 alpha/beta hydrolase -
FOH49_RS26350 (FOH49_026395) 5049721..5050662 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FOH49_RS26355 (FOH49_026400) 5050707..5051144 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FOH49_RS26360 (FOH49_026405) 5051141..5052001 - 861 WP_023280688.1 virulence factor BrkB family protein -
FOH49_RS26365 (FOH49_026410) 5051995..5052594 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T159661 WP_002882817.1 NZ_CP054406:c5048343-5047960 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T159661 NZ_CP070103:2471034-2471432 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT159661 WP_004150355.1 NZ_CP054406:c5048585-5048343 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT159661 NZ_CP070103:2470738-2471031 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References