Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3874556..3875175 Replicon chromosome
Accession NZ_CP054406
Organism Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FOH49_RS20745 Protein ID WP_002892050.1
Coordinates 3874957..3875175 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FOH49_RS20740 Protein ID WP_002892066.1
Coordinates 3874556..3874930 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH49_RS20730 (FOH49_020760) 3869708..3870901 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FOH49_RS20735 (FOH49_020765) 3870924..3874070 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
FOH49_RS20740 (FOH49_020770) 3874556..3874930 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FOH49_RS20745 (FOH49_020775) 3874957..3875175 + 219 WP_002892050.1 HHA domain-containing protein Toxin
FOH49_RS20750 (FOH49_020780) 3875334..3875900 + 567 WP_002892030.1 maltose O-acetyltransferase -
FOH49_RS20755 (FOH49_020785) 3875872..3876012 - 141 WP_004147370.1 hypothetical protein -
FOH49_RS20760 (FOH49_020790) 3876033..3876503 + 471 WP_002892026.1 YlaC family protein -
FOH49_RS20765 (FOH49_020795) 3876478..3877929 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
FOH49_RS20770 (FOH49_020800) 3878030..3878728 + 699 WP_002892021.1 GNAT family protein -
FOH49_RS20775 (FOH49_020805) 3878725..3878865 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FOH49_RS20780 (FOH49_020810) 3878865..3879128 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T159657 WP_002892050.1 NZ_CP054406:3874957-3875175 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T159657 NZ_CP070103:1912287-1912460 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT159657 WP_002892066.1 NZ_CP054406:3874556-3874930 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT159657 NZ_CP070103:1911963-1912259 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References