Detailed information of TA system TA159641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 115609..116345
• Replicon: plasmid pKC141K
• Accession: NZ_CP054404
• Organism: Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: FOH49_RS00575
• Protein ID: WP_004187044.1
• Coordinates: 115863..116345 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FOH49_RS00570
• Protein ID: WP_003026799.1
• Coordinates: 115609..115875 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH49_RS00550 (FOH49_000550)	110758..111738	+	981	Protein_111	IS5-like element ISKpn26 family transposase	-
FOH49_RS00555 (FOH49_000555)	111796..112041	+	246	WP_032238678.1	hypothetical protein	-
FOH49_RS00560 (FOH49_000560)	112010..114595	+	2586	WP_004187040.1	EAL domain-containing protein	-
FOH49_RS00565 (FOH49_000565)	114754..115458	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH49_RS00570 (FOH49_000570)	115609..115875	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FOH49_RS00575 (FOH49_000575)	115863..116345	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
FOH49_RS00580 (FOH49_000580)	116553..117899	+	1347	WP_077250283.1	ISNCY family transposase	-
FOH49_RS00585 (FOH49_000585)	117958..118752	+	795	WP_004187050.1	tetratricopeptide repeat protein	-
FOH49_RS00590 (FOH49_000590)	118791..119135	-	345	WP_156651253.1	hypothetical protein	-
FOH49_RS00595 (FOH49_000595)	119244..120169	-	926	Protein_120	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..141398	141398
	-	inside	IScluster/Tn	-	-	110758..120214	9456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T159641 WP_004187044.1 NZ_CP054404:115863-116345 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T159641 NZ_CP070098:4884421-4884771 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT159641 WP_003026799.1 NZ_CP054404:115609-115875 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT159641 NZ_CP070098:4884176-4884427 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66