Detailed information of TA system TA159640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 102212..102734
• Replicon: plasmid pKC141K
• Accession: NZ_CP054404
• Organism: Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: FOH49_RS00485
• Protein ID: WP_004187019.1
• Coordinates: 102212..102496 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: FOH49_RS00490
• Protein ID: WP_004187025.1
• Coordinates: 102486..102734 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH49_RS00460 (FOH49_000465)	98374..98787	+	414	WP_004187013.1	hypothetical protein	-
FOH49_RS00465 (FOH49_000470)	98879..99847	+	969	WP_080465744.1	IS5 family transposase	-
FOH49_RS00475	100508..100759	+	252	Protein_95	conjugal transfer protein TraH	-
FOH49_RS00480 (FOH49_000480)	100841..102196	+	1356	WP_004187017.1	ISNCY family transposase	-
FOH49_RS00485 (FOH49_000485)	102212..102496	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH49_RS00490 (FOH49_000490)	102486..102734	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
FOH49_RS00495 (FOH49_000495)	103024..103448	-	425	Protein_99	IS1 family transposase	-
FOH49_RS00500 (FOH49_000500)	103636..103752	-	117	Protein_100	transposase domain-containing protein	-
FOH49_RS00505 (FOH49_000505)	103697..103960	-	264	WP_004187027.1	transposase	-
FOH49_RS00510 (FOH49_000510)	103975..104238	+	264	WP_004118208.1	hypothetical protein	-
FOH49_RS00515 (FOH49_000515)	104462..104743	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
FOH49_RS00520 (FOH49_000520)	104778..105347	+	570	WP_004118809.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..141398	141398
	-	inside	IScluster/Tn	-	-	95212..103254	8042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T159640 WP_004187019.1 NZ_CP054404:c102496-102212 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T159640 NZ_CP070098:c4733975-4733598 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT159640 WP_004187025.1 NZ_CP054404:c102734-102486 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT159640 NZ_CP070098:c4734433-4734065 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4