Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5047987..5048612 Replicon chromosome
Accession NZ_CP054403
Organism Klebsiella pneumoniae strain KCJ3K292

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FOH48_RS26405 Protein ID WP_002882817.1
Coordinates 5047987..5048370 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FOH48_RS26410 Protein ID WP_004150355.1
Coordinates 5048370..5048612 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH48_RS26390 (FOH48_026305) 5045353..5046255 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FOH48_RS26395 (FOH48_026310) 5046252..5046887 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FOH48_RS26400 (FOH48_026315) 5046884..5047813 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FOH48_RS26405 (FOH48_026320) 5047987..5048370 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FOH48_RS26410 (FOH48_026325) 5048370..5048612 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FOH48_RS26415 (FOH48_026330) 5048817..5049734 + 918 WP_004181612.1 alpha/beta hydrolase -
FOH48_RS26420 (FOH48_026335) 5049748..5050689 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FOH48_RS26425 (FOH48_026340) 5050734..5051171 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FOH48_RS26430 (FOH48_026345) 5051168..5052028 - 861 WP_023280688.1 virulence factor BrkB family protein -
FOH48_RS26435 (FOH48_026350) 5052022..5052621 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T159639 WP_002882817.1 NZ_CP054403:c5048370-5047987 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T159639 NZ_CP070098:4722477-4722650 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT159639 WP_004150355.1 NZ_CP054403:c5048612-5048370 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT159639 NZ_CP070098:4722153-4722449 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References