Detailed information of TA system TA159619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 42343..43079
• Replicon: plasmid pKC141K
• Accession: NZ_CP054401
• Organism: Klebsiella pneumoniae strain KCJ3K292

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: FOH48_RS00225
• Protein ID: WP_004187044.1
• Coordinates: 42597..43079 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FOH48_RS00220
• Protein ID: WP_003026799.1
• Coordinates: 42343..42609 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH48_RS00200 (FOH48_000195)	37492..38472	+	981	Protein_39	IS5-like element ISKpn26 family transposase	-
FOH48_RS00205 (FOH48_000200)	38530..38775	+	246	WP_032238678.1	hypothetical protein	-
FOH48_RS00210 (FOH48_000205)	38744..41329	+	2586	WP_004187040.1	EAL domain-containing protein	-
FOH48_RS00215 (FOH48_000210)	41488..42192	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH48_RS00220 (FOH48_000215)	42343..42609	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FOH48_RS00225 (FOH48_000220)	42597..43079	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
FOH48_RS00230 (FOH48_000225)	43287..44633	+	1347	WP_077250283.1	ISNCY family transposase	-
FOH48_RS00235 (FOH48_000230)	44692..45486	+	795	WP_004187050.1	tetratricopeptide repeat protein	-
FOH48_RS00240 (FOH48_000235)	45525..45869	-	345	WP_156651253.1	hypothetical protein	-
FOH48_RS00245 (FOH48_000240)	45978..46946	-	969	WP_080465744.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..141474	141474
	-	inside	IScluster/Tn	-	-	37492..46946	9454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T159619 WP_004187044.1 NZ_CP054401:42597-43079 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T159619 NZ_CP070098:c393261-393043 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT159619 WP_003026799.1 NZ_CP054401:42343-42609 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT159619 NZ_CP070098:c393661-393287 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66