Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5047988..5048613 Replicon chromosome
Accession NZ_CP054400
Organism Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FOH51_RS26335 Protein ID WP_002882817.1
Coordinates 5047988..5048371 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FOH51_RS26340 Protein ID WP_004150355.1
Coordinates 5048371..5048613 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH51_RS26320 (FOH51_026305) 5045354..5046256 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FOH51_RS26325 (FOH51_026310) 5046253..5046888 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FOH51_RS26330 (FOH51_026315) 5046885..5047814 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FOH51_RS26335 (FOH51_026320) 5047988..5048371 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FOH51_RS26340 (FOH51_026325) 5048371..5048613 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FOH51_RS26345 (FOH51_026330) 5048818..5049735 + 918 WP_004181612.1 alpha/beta hydrolase -
FOH51_RS26350 (FOH51_026335) 5049749..5050690 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FOH51_RS26355 (FOH51_026340) 5050735..5051172 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FOH51_RS26360 (FOH51_026345) 5051169..5052029 - 861 WP_023280688.1 virulence factor BrkB family protein -
FOH51_RS26365 (FOH51_026350) 5052023..5052622 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T159617 WP_002882817.1 NZ_CP054400:c5048371-5047988 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T159617 NZ_CP070098:171577-171975 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT159617 WP_004150355.1 NZ_CP054400:c5048613-5048371 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT159617 NZ_CP070098:171281-171574 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References