Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4461740..4462550 Replicon chromosome
Accession NZ_CP054400
Organism Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag FOH51_RS23575 Protein ID WP_004178461.1
Coordinates 4461740..4462273 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag FOH51_RS23580 Protein ID WP_002887278.1
Coordinates 4462284..4462550 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOH51_RS23570 (FOH51_023560) 4460571..4461692 + 1122 WP_004186850.1 cupin domain-containing protein -
FOH51_RS23575 (FOH51_023565) 4461740..4462273 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
FOH51_RS23580 (FOH51_023570) 4462284..4462550 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
FOH51_RS23585 (FOH51_023575) 4462653..4464086 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
FOH51_RS23590 (FOH51_023580) 4464076..4464759 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
FOH51_RS23595 (FOH51_023585) 4464932..4466317 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
FOH51_RS23600 (FOH51_023590) 4466335..4466679 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T159614 WP_004178461.1 NZ_CP054400:c4462273-4461740 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T159614 NZ_CP070092:c4565971-4565869 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT159614 WP_002887278.1 NZ_CP054400:c4462550-4462284 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT159614 NZ_CP070092:4565862-4566007 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References