Detailed information of TA system TA159601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 776722..777500
• Replicon: chromosome
• Accession: NZ_CP054400
• Organism: Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: FOH51_RS05535
• Protein ID: WP_031591140.1
• Coordinates: 777012..777500 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: FOH51_RS05530
• Protein ID: WP_004150912.1
• Coordinates: 776722..777015 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH51_RS05510 (FOH51_005505)	771930..772532	-	603	WP_004174410.1	short chain dehydrogenase	-
FOH51_RS05515 (FOH51_005510)	772630..773541	+	912	WP_004181308.1	LysR family transcriptional regulator	-
FOH51_RS05520 (FOH51_005515)	773542..774690	-	1149	WP_004174416.1	PLP-dependent aspartate aminotransferase family protein	-
FOH51_RS05525 (FOH51_005520)	774701..776077	-	1377	WP_004217775.1	cystathionine beta-synthase	-
FOH51_RS05530 (FOH51_005525)	776722..777015	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
FOH51_RS05535 (FOH51_005530)	777012..777500	+	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
FOH51_RS05540 (FOH51_005535)	777581..781324	-	3744	WP_144296106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T159601 WP_031591140.1 NZ_CP054400:777012-777500 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T159601 NZ_CP070088:c3588243-3587860 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT159601 WP_004150912.1 NZ_CP054400:776722-777015 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT159601 NZ_CP070088:c3588485-3588243 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4