Detailed information of TA system TA159597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 67731..68467
• Replicon: plasmid pKC141K
• Accession: NZ_CP054398
• Organism: Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: FOH51_RS00345
• Protein ID: WP_004187044.1
• Coordinates: 67985..68467 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FOH51_RS00340
• Protein ID: WP_003026799.1
• Coordinates: 67731..67997 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH51_RS00320 (FOH51_000315)	62880..63860	+	981	Protein_63	IS5-like element ISKpn26 family transposase	-
FOH51_RS00325 (FOH51_000320)	63918..64163	+	246	WP_032238678.1	hypothetical protein	-
FOH51_RS00330 (FOH51_000325)	64132..66717	+	2586	WP_004187040.1	EAL domain-containing protein	-
FOH51_RS00335 (FOH51_000330)	66876..67580	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH51_RS00340 (FOH51_000335)	67731..67997	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FOH51_RS00345 (FOH51_000340)	67985..68467	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
FOH51_RS00350 (FOH51_000345)	68675..70021	+	1347	WP_077250283.1	ISNCY family transposase	-
FOH51_RS00355 (FOH51_000350)	70080..70874	+	795	WP_004187050.1	tetratricopeptide repeat protein	-
FOH51_RS00360 (FOH51_000355)	70913..71257	-	345	WP_156651253.1	hypothetical protein	-
FOH51_RS00365 (FOH51_000360)	71366..72334	-	969	WP_080465744.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..141474	141474
	-	inside	IScluster/Tn	-	-	62880..72334	9454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T159597 WP_004187044.1 NZ_CP054398:67985-68467 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T159597 NZ_CP070088:c2983138-2982605 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCTGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT159597 WP_003026799.1 NZ_CP054398:67731-67997 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT159597 NZ_CP070088:c2983415-2983149 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66