Detailed information of TA system TA159596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 54334..54856
• Replicon: plasmid pKC141K
• Accession: NZ_CP054398
• Organism: Klebsiella pneumoniae strain KCJ3K307

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: FOH51_RS00255
• Protein ID: WP_004187019.1
• Coordinates: 54334..54618 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: FOH51_RS00260
• Protein ID: WP_004187025.1
• Coordinates: 54608..54856 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH51_RS00230 (FOH51_000230)	50495..50908	+	414	WP_004187013.1	hypothetical protein	-
FOH51_RS00235 (FOH51_000235)	51001..51969	+	969	WP_080465744.1	IS5 family transposase	-
FOH51_RS00245	52630..52881	+	252	Protein_47	conjugal transfer protein TraH	-
FOH51_RS00250 (FOH51_000245)	52963..54318	+	1356	WP_004187017.1	ISNCY family transposase	-
FOH51_RS00255 (FOH51_000250)	54334..54618	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH51_RS00260 (FOH51_000255)	54608..54856	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
FOH51_RS00265 (FOH51_000260)	55146..55570	-	425	Protein_51	IS1 family transposase	-
FOH51_RS00270 (FOH51_000265)	55758..55874	-	117	Protein_52	transposase domain-containing protein	-
FOH51_RS00275 (FOH51_000270)	55819..56082	-	264	WP_004187027.1	transposase	-
FOH51_RS00280 (FOH51_000275)	56097..56360	+	264	WP_004118208.1	hypothetical protein	-
FOH51_RS00285 (FOH51_000280)	56584..56865	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
FOH51_RS00290 (FOH51_000285)	56900..57469	+	570	WP_004118809.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkC / mrkB / mrkA	1..141474	141474
	-	inside	IScluster/Tn	-	-	47333..55376	8043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T159596 WP_004187019.1 NZ_CP054398:c54618-54334 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T159596 NZ_CP070088:2392511-2392729 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT159596 WP_004187025.1 NZ_CP054398:c54856-54608 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT159596 NZ_CP070088:2392110-2392484 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ATATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4