159582

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2919855..2920692 Replicon chromosome
Accession NZ_CP054388
Organism Escherichia coli strain 1517k

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag E2126_RS13850 Protein ID WP_000227782.1
Coordinates 2920150..2920692 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag E2126_RS13845 Protein ID WP_001353405.1
Coordinates 2919855..2920166 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2126_RS13820 2914875..2915822 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
E2126_RS13825 2915844..2917835 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
E2126_RS13830 2917825..2918439 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
E2126_RS13835 2918439..2918768 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
E2126_RS13840 2918780..2919670 + 891 WP_000971327.1 heme o synthase -
E2126_RS13845 2919855..2920166 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
E2126_RS13850 2920150..2920692 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
E2126_RS13855 2920748..2921592 - 845 Protein_2711 sel1 repeat family protein -
E2126_RS13860 2921999..2923363 + 1365 WP_001000978.1 MFS transporter -
E2126_RS13865 2923491..2923982 - 492 WP_001138904.1 nucleotide binding protein YajQ -
E2126_RS13870 2924150..2925061 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
E2126_RS13875 2925024..2925614 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T159582 WP_000227782.1 NZ_CP054388:2920150-2920692 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T159582 NZ_CP070087:c1242158-1241874 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT159582 WP_001353405.1 NZ_CP054388:2919855-2920166 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT159582 NZ_CP070087:c1242390-1242148 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References