Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4375116..4375916 Replicon chromosome
Accession NZ_CP054371
Organism Escherichia coli strain SCU-316

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag HPE41_RS20675 Protein ID WP_000342447.1
Coordinates 4375116..4375643 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag HPE41_RS20680 Protein ID WP_001277109.1
Coordinates 4375650..4375916 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE41_RS20650 4370191..4370958 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HPE41_RS20655 4370955..4372232 - 1278 WP_000803778.1 branched chain amino acid ABC transporter permease LivM -
HPE41_RS20660 4372229..4373155 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HPE41_RS20665 4373203..4374312 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HPE41_RS20670 4374736..4375119 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
HPE41_RS20675 4375116..4375643 - 528 WP_000342447.1 GNAT family N-acetyltransferase Toxin
HPE41_RS20680 4375650..4375916 - 267 WP_001277109.1 DUF1778 domain-containing protein Antitoxin
HPE41_RS20685 4376066..4377169 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HPE41_RS20690 4377440..4378294 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HPE41_RS20695 4378539..4379597 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
HPE41_RS20700 4379590..4380258 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T159562 WP_000342447.1 NZ_CP054371:c4375643-4375116 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T159562 NZ_CP070077:2361711-2361818 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT159562 WP_001277109.1 NZ_CP054371:c4375916-4375650 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp

>AT159562 NZ_CP070077:c2361653-2361599 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References