Detailed information of TA system TA159561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4328070..4328824
• Replicon: chromosome
• Accession: NZ_CP054371
• Organism: Escherichia coli strain SCU-316

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HPE41_RS20475
• Protein ID: WP_001301452.1
• Coordinates: 4328339..4328824 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HPE41_RS20470
• Protein ID: WP_000801914.1
• Coordinates: 4328070..4328348 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE41_RS20465	4325298..4328003	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
HPE41_RS20470	4328070..4328348	+	279	WP_000801914.1	DUF1778 domain-containing protein	Antitoxin
HPE41_RS20475	4328339..4328824	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
HPE41_RS20480	4328874..4329902	-	1029	WP_024257792.1	RNA 3'-terminal phosphate cyclase	-
HPE41_RS20485	4329906..4331132	-	1227	WP_001105465.1	RNA-splicing ligase RtcB	-
HPE41_RS20490	4331319..4332917	+	1599	WP_071337529.1	DNA-binding transcriptional regulator RtcR	-
HPE41_RS20495	4332899..4333657	-	759	WP_000815099.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T159561 WP_001301452.1 NZ_CP054371:4328339-4328824 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T159561 NZ_CP070077:c1936156-1935845 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.91 Da
• Isoelectric Point: 4.6336

>AT159561 WP_000801914.1 NZ_CP054371:4328070-4328348 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFIVDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT159561 NZ_CP070077:c1935844-1935554 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG