Detailed information of TA system TA159558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3650603..3651186
• Replicon: chromosome
• Accession: NZ_CP054371
• Organism: Escherichia coli strain SCU-316

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U9Y5H3
• Locus tag: HPE41_RS17205
• Protein ID: WP_000254735.1
• Coordinates: 3650603..3650938 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U9Y5F1
• Locus tag: HPE41_RS17210
• Protein ID: WP_000581936.1
• Coordinates: 3650938..3651186 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE41_RS17190	3646489..3647787	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
HPE41_RS17195	3647875..3649512	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HPE41_RS17200	3649740..3650531	-	792	WP_001071673.1	nucleoside triphosphate pyrophosphohydrolase	-
HPE41_RS17205	3650603..3650938	-	336	WP_000254735.1	endoribonuclease MazF	Toxin
HPE41_RS17210	3650938..3651186	-	249	WP_000581936.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HPE41_RS17215	3651264..3653498	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
HPE41_RS17220	3653546..3654847	-	1302	WP_000046797.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12070.03 Da
• Isoelectric Point: 8.2605

>T159558 WP_000254735.1 NZ_CP054371:c3650938-3650603 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRAKGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T159558 NZ_CP070077:c1493051-1492701 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9341.71 Da
• Isoelectric Point: 4.4536

>AT159558 WP_000581936.1 NZ_CP054371:c3651186-3650938 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHDNIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT159558 NZ_CP070077:c1493296-1493045 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: U9Y5H3

Antitoxin

• AlphaFold DB: U9Y5F1