159557

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3353831..3354456 Replicon chromosome
Accession NZ_CP054371
Organism Escherichia coli strain SCU-316

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HPE41_RS15785 Protein ID WP_000911329.1
Coordinates 3353831..3354229 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HPE41_RS15790 Protein ID WP_000450524.1
Coordinates 3354229..3354456 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE41_RS15765 3349738..3349938 + 201 WP_000383836.1 YpfN family protein -
HPE41_RS15770 3350019..3350717 - 699 WP_000679812.1 esterase -
HPE41_RS15775 3350791..3352806 - 2016 WP_000829353.1 tRNA cytosine(34) acetyltransferase TmcA -
HPE41_RS15780 3352821..3353684 - 864 WP_001267498.1 neutral zinc metallopeptidase -
HPE41_RS15785 3353831..3354229 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HPE41_RS15790 3354229..3354456 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HPE41_RS15795 3354611..3355324 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HPE41_RS15800 3355537..3356571 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HPE41_RS15805 3356588..3357466 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HPE41_RS15810 3357612..3358184 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HPE41_RS15815 3358184..3358654 + 471 WP_001068690.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T159557 WP_000911329.1 NZ_CP054371:c3354229-3353831 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T159557 NZ_CP070077:c1481450-1481160 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT159557 WP_000450524.1 NZ_CP054371:c3354456-3354229 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT159557 NZ_CP070077:c1481682-1481440 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References