Detailed information of TA system TA159547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2380360..2380886
• Replicon: chromosome
• Accession: NZ_CP054371
• Organism: Escherichia coli strain SCU-316

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HPE41_RS11110
• Protein ID: WP_000323025.1
• Coordinates: 2380360..2380647 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HPE41_RS11115
• Protein ID: WP_000534858.1
• Coordinates: 2380647..2380886 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE41_RS11065	2375383..2375598	-	216	WP_000839590.1	phage lysis protein EssD	-
HPE41_RS11070	2376352..2376567	-	216	WP_000066484.1	cold shock-like protein CspB	-
HPE41_RS11075	2376868..2377080	+	213	WP_000087756.1	cold shock-like protein CspF	-
HPE41_RS11080	2377135..2377224	+	90	WP_120795389.1	hypothetical protein	-
HPE41_RS11085	2377503..2378255	-	753	WP_112033706.1	antitermination protein	-
HPE41_RS11090	2378269..2379318	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
HPE41_RS11095	2379320..2379598	-	279	WP_012304870.1	hypothetical protein	-
HPE41_RS11100	2379665..2379916	-	252	WP_000980994.1	hypothetical protein	-
HPE41_RS11105	2380133..2380288	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HPE41_RS11110	2380360..2380647	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HPE41_RS11115	2380647..2380886	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HPE41_RS11120	2380911..2381216	+	306	WP_001326990.1	hypothetical protein	-
HPE41_RS11125	2381419..2381751	+	333	WP_001301033.1	protein FlxA	-
HPE41_RS11130	2382188..2383501	-	1314	WP_000589005.1	ISNCY family transposase	-
HPE41_RS11135	2383679..2383861	+	183	WP_000955178.1	hypothetical protein	-
HPE41_RS11140	2385172..2385528	-	357	WP_174166959.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2333899..2394137	60238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T159547 WP_000323025.1 NZ_CP054371:c2380647-2380360 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T159547 NZ_CP070072:c4238-3840 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT159547 WP_000534858.1 NZ_CP054371:c2380886-2380647 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT159547 NZ_CP070072:c4465-4238 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8