Detailed information of TA system TA159545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2201059..2201697
• Replicon: chromosome
• Accession: NZ_CP054371
• Organism: Escherichia coli strain SCU-316

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A080J2T0
• Locus tag: HPE41_RS10245
• Protein ID: WP_000813793.1
• Coordinates: 2201059..2201235 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HPE41_RS10250
• Protein ID: WP_001270286.1
• Coordinates: 2201281..2201697 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE41_RS10225	2196681..2197892	-	1212	WP_023154256.1	BenE family transporter YdcO	-
HPE41_RS10230	2197945..2198481	+	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
HPE41_RS10235	2198554..2200515	+	1962	WP_024257894.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HPE41_RS10240	2200607..2200837	-	231	WP_000494244.1	YncJ family protein	-
HPE41_RS10245	2201059..2201235	+	177	WP_000813793.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HPE41_RS10250	2201281..2201697	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HPE41_RS10255	2201776..2203182	+	1407	WP_000760655.1	PLP-dependent aminotransferase family protein	-
HPE41_RS10260	2203427..2204572	+	1146	WP_000047419.1	ABC transporter substrate-binding protein	-
HPE41_RS10265	2204590..2205603	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
HPE41_RS10270	2205604..2206545	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.83 Da
• Isoelectric Point: 10.9223

>T159545 WP_000813793.1 NZ_CP054371:2201059-2201235 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGMRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T159545 NZ_CP070071:c4690406-4690029 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT159545 WP_001270286.1 NZ_CP054371:2201281-2201697 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT159545 NZ_CP070071:c4690864-4690496 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A080J2T0