Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 646893..647443 Replicon chromosome
Accession NZ_CP054371
Organism Escherichia coli strain SCU-316

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag HPE41_RS03090 Protein ID WP_001160974.1
Coordinates 647129..647443 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HPE41_RS03085 Protein ID WP_000125566.1
Coordinates 646893..647126 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE41_RS03060 641965..642252 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HPE41_RS03065 642311..643642 + 1332 WP_089610134.1 MFS transporter -
HPE41_RS03070 643750..644280 + 531 WP_072686445.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HPE41_RS03075 644273..646135 + 1863 WP_089610132.1 glutathione-regulated potassium-efflux system protein KefC -
HPE41_RS03080 646328..646807 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HPE41_RS03085 646893..647126 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HPE41_RS03090 647129..647443 + 315 WP_001160974.1 CcdB family protein Toxin
HPE41_RS03095 647440..648288 - 849 WP_000257188.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HPE41_RS03100 648295..648672 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HPE41_RS03105 648675..649496 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HPE41_RS03110 649493..650482 - 990 WP_089610127.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HPE41_RS03115 650482..651768 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T159538 WP_001160974.1 NZ_CP054371:647129-647443 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T159538 NZ_CP070071:c3882597-3882220 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT159538 WP_000125566.1 NZ_CP054371:646893-647126 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT159538 NZ_CP070071:c3883055-3882687 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References