Detailed information of TA system TA159527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 82357..82892
• Replicon: plasmid pSCU-115-1
• Accession: NZ_CP054369
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T1PX77
• Locus tag: HPE48_RS23895
• Protein ID: WP_001523468.1
• Coordinates: 82357..82644 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: HPE48_RS23900
• Protein ID: WP_001139206.1
• Coordinates: 82641..82892 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS23875	77808..78572	-	765	WP_001523453.1	helix-turn-helix domain-containing protein	-
HPE48_RS23880	79575..80168	+	594	WP_001523457.1	hypothetical protein	-
HPE48_RS23885	80448..81035	-	588	WP_024189343.1	serine acetyltransferase	-
HPE48_RS23890	81802..82347	-	546	WP_001523466.1	hypothetical protein	-
HPE48_RS23895	82357..82644	-	288	WP_001523468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPE48_RS23900	82641..82892	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HPE48_RS23905	82979..83146	-	168	WP_032146603.1	hypothetical protein	-
HPE48_RS23910	83848..84705	-	858	WP_001523472.1	incFII family plasmid replication initiator RepA	-
HPE48_RS23915	84698..85180	-	483	WP_001523473.1	hypothetical protein	-
HPE48_RS23920	85173..85247	-	75	WP_001442103.1	RepA leader peptide Tap	-
HPE48_RS23925	85481..85738	-	258	WP_000083845.1	replication regulatory protein RepA	-
HPE48_RS23930	86022..86228	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	-
-	86215..86276	+	62	NuclAT_1	-	-
-	86215..86276	+	62	NuclAT_1	-	-
-	86215..86276	+	62	NuclAT_1	-	-
-	86215..86276	+	62	NuclAT_1	-	-
HPE48_RS23935	86859..87071	-	213	WP_032084501.1	hypothetical protein	-
HPE48_RS23940	87204..87764	-	561	WP_001523482.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / hlyB / iroB / iroC / iroD / iroE / iroN / papC / papH	1..148443	148443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.18 Da
• Isoelectric Point: 10.6741

>T159527 WP_001523468.1 NZ_CP054369:c82644-82357 [Escherichia coli]
MTYTVKFRDDALREWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T159527 NZ_CP070071:1405351-1406667 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGATCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAACAGCTCACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGTGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGCAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACCGTCGGTTAAGCCGAGAATGGTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT159527 WP_001139206.1 NZ_CP054369:c82892-82641 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT159527 NZ_CP070071:1405085-1405351 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACCTTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: T1PX77

Antitoxin

• AlphaFold DB: T1PXV2