Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4021651..4022305 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag HPE48_RS19520 Protein ID WP_174144107.1
Coordinates 4021898..4022305 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HPE48_RS19515 Protein ID WP_000354046.1
Coordinates 4021651..4021917 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS19495 4017739..4019172 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
HPE48_RS19500 4019217..4019528 + 312 WP_174144106.1 N(4)-acetylcytidine aminohydrolase -
HPE48_RS19505 4019692..4020345 + 654 WP_000250273.1 hemolysin III family protein -
HPE48_RS19510 4020428..4021408 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
HPE48_RS19515 4021651..4021917 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HPE48_RS19520 4021898..4022305 + 408 WP_174144107.1 protein YgfX Toxin
HPE48_RS19525 4022345..4022866 - 522 WP_001055874.1 flavodoxin FldB -
HPE48_RS19530 4022978..4023874 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HPE48_RS19535 4023899..4024609 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HPE48_RS19540 4024615..4026348 + 1734 WP_174144108.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16143.09 Da        Isoelectric Point: 11.7064

>T159526 WP_174144107.1 NZ_CP054368:4021898-4022305 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDRRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159526 NZ_CP070071:c1023857-1023663 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACGGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159526 WP_000354046.1 NZ_CP054368:4021651-4021917 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159526 NZ_CP070071:1023486-1023664 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCATTTAGTAGGAGTTCCACCTTCTTTTT
CAATAGTGGTAGTTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References