Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3416511..3417311 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag HPE48_RS16525 Protein ID WP_000342452.1
Coordinates 3416784..3417311 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag HPE48_RS16520 Protein ID WP_001277107.1
Coordinates 3416511..3416777 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS16500 3412171..3412839 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HPE48_RS16505 3412832..3413890 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HPE48_RS16510 3414134..3414988 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HPE48_RS16515 3415259..3416362 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HPE48_RS16520 3416511..3416777 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
HPE48_RS16525 3416784..3417311 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
HPE48_RS16530 3417308..3417691 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
HPE48_RS16535 3418115..3419224 + 1110 WP_001312159.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HPE48_RS16540 3419272..3420198 + 927 WP_174144040.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HPE48_RS16545 3420195..3421472 + 1278 WP_000803817.1 branched chain amino acid ABC transporter permease LivM -
HPE48_RS16550 3421469..3422236 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T159523 WP_000342452.1 NZ_CP054368:3416784-3417311 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T159523 NZ_CP070071:769689-769940 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT159523 WP_001277107.1 NZ_CP054368:3416511-3416777 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT159523 NZ_CP070071:769976-770191 [Escherichia coli]
CTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGATCAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGA
AGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAGGTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGG
TATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGTCGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References