Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2190197..2190747 Replicon chromosome
Accession NZ_CP054368
Organism Escherichia coli strain SCU-115

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HPE48_RS10855 Protein ID WP_001160966.1
Coordinates 2190197..2190511 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HPE48_RS10860 Protein ID WP_000125566.1
Coordinates 2190514..2190747 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE48_RS10830 2185872..2187158 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HPE48_RS10835 2187158..2188147 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HPE48_RS10840 2188144..2188965 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HPE48_RS10845 2188968..2189345 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HPE48_RS10850 2189352..2190200 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HPE48_RS10855 2190197..2190511 - 315 WP_001160966.1 CcdB family protein Toxin
HPE48_RS10860 2190514..2190747 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HPE48_RS10865 2190833..2191312 - 480 WP_089536762.1 type 3 dihydrofolate reductase -
HPE48_RS10870 2191504..2193366 - 1863 WP_001523933.1 glutathione-regulated potassium-efflux system protein KefC -
HPE48_RS10875 2193359..2193889 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HPE48_RS10880 2193997..2195328 - 1332 WP_001523932.1 MFS transporter -
HPE48_RS10885 2195387..2195674 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T159516 WP_001160966.1 NZ_CP054368:c2190511-2190197 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T159516 NZ_CP070066:c4793757-4793446 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT159516 WP_000125566.1 NZ_CP054368:c2190747-2190514 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT159516 NZ_CP070066:c4793445-4793155 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References