Detailed information of TA system TA159512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 771055..772643
• Replicon: chromosome
• Accession: NZ_CP054368
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HPE48_RS04075
• Protein ID: WP_174143733.1
• Coordinates: 771321..772643 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: HPE48_RS04070
• Protein ID: WP_001296076.1
• Coordinates: 771055..771321 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS04055	767423..768874	+	1452	WP_096856683.1	tagaturonate reductase	-
HPE48_RS04060	769071..769985	+	915	WP_001385356.1	bestrophin family protein	-
HPE48_RS04065	769989..770747	-	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
HPE48_RS04070	771055..771321	+	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HPE48_RS04075	771321..772643	+	1323	WP_174143733.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HPE48_RS04080	773555..774118	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
HPE48_RS04085	774480..775190	+	711	WP_001195177.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49283.98 Da
• Isoelectric Point: 8.1297

>T159512 WP_174143733.1 NZ_CP054368:771321-772643 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQAFGLPSSVKYESDGGPGITQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKISLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T159512 NZ_CP070066:c3886680-3886282 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT159512 WP_001296076.1 NZ_CP054368:771055-771321 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT159512 NZ_CP070066:c3886976-3886683 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A061YNW4