Detailed information of TA system TA159511

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 453953..454785
• Replicon: chromosome
• Accession: NZ_CP054368
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0D8W767
• Locus tag: HPE48_RS02350
• Protein ID: WP_000854697.1
• Coordinates: 453953..454327 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0D8WAR2
• Locus tag: HPE48_RS02355
• Protein ID: WP_001354195.1
• Coordinates: 454417..454785 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS02320	449103..449756	-	654	WP_001296116.1	ATP-binding cassette domain-containing protein	-
HPE48_RS02325	449756..451291	-	1536	WP_174143700.1	ABC transporter permease	-
HPE48_RS02330	451264..452430	-	1167	WP_001524787.1	ABC transporter substrate-binding protein	-
HPE48_RS02335	452650..453446	-	797	Protein_454	DUF4942 domain-containing protein	-
HPE48_RS02340	453531..453728	-	198	WP_085975623.1	DUF957 domain-containing protein	-
HPE48_RS02345	453804..453956	-	153	Protein_456	hypothetical protein	-
HPE48_RS02350	453953..454327	-	375	WP_000854697.1	TA system toxin CbtA family protein	Toxin
HPE48_RS02355	454417..454785	-	369	WP_001354195.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPE48_RS02360	454835..455479	-	645	Protein_459	antitoxin of toxin-antitoxin stability system	-
HPE48_RS02365	455498..455719	-	222	WP_000692303.1	DUF987 domain-containing protein	-
HPE48_RS02370	455782..456258	-	477	WP_174143701.1	RadC family protein	-
HPE48_RS02375	456274..456753	-	480	WP_000860074.1	antirestriction protein	-
HPE48_RS02380	456835..457653	-	819	WP_001234688.1	DUF945 domain-containing protein	-
HPE48_RS02385	457754..457906	-	153	WP_001696589.1	DUF905 family protein	-
HPE48_RS02390	457912..458589	-	678	WP_001097365.1	hypothetical protein	-
HPE48_RS02395	458708..459592	-	885	WP_001696588.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14023.07 Da
• Isoelectric Point: 7.1881

>T159511 WP_000854697.1 NZ_CP054368:c454327-453953 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIELHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T159511 NZ_CP070066:3687172-3687390 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13708.48 Da
• Isoelectric Point: 6.8270

>AT159511 WP_001354195.1 NZ_CP054368:c454785-454417 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLPMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT159511 NZ_CP070066:3686772-3687146 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0D8W767

Antitoxin

• AlphaFold DB: A0A0D8WAR2