Detailed information of TA system TA159496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4721434..4722139
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HPE49_RS22875
• Protein ID: WP_000539521.1
• Coordinates: 4721753..4722139 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HPE49_RS22870
• Protein ID: WP_001280945.1
• Coordinates: 4721434..4721763 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS22855	4716591..4717502	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
HPE49_RS22860	4717680..4720028	+	2349	WP_174144561.1	EAL domain-containing protein	-
HPE49_RS22865	4720036..4721364	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
HPE49_RS22870	4721434..4721763	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HPE49_RS22875	4721753..4722139	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPE49_RS22880	4722365..4723690	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HPE49_RS22885	4723903..4724286	+	384	WP_000497137.1	biofilm formation regulator BssR	-
HPE49_RS22890	4724397..4725512	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
HPE49_RS22895	4725509..4726135	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T159496 WP_000539521.1 NZ_CP054363:c4722139-4721753 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T159496 NZ_CP070064:c31576-31295 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGCGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT159496 WP_001280945.1 NZ_CP054363:c4721763-4721434 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT159496 NZ_CP070064:c31817-31566 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6