Detailed information of TA system TA159492

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3675844..3676551
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A4T3Q6D2
• Locus tag: HPE49_RS17715
• Protein ID: WP_001683515.1
• Coordinates: 3676210..3676551 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HPE49_RS17710
• Protein ID: WP_001296928.1
• Coordinates: 3675844..3676179 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS17685	3671791..3672009	-	219	WP_042106713.1	AlpA family transcriptional regulator	-
HPE49_RS17690	3672127..3672741	-	615	WP_000772910.1	inovirus Gp2 family protein	-
HPE49_RS17695	3673334..3674286	+	953	Protein_3453	hypothetical protein	-
HPE49_RS17700	3674523..3675341	+	819	WP_024223204.1	DUF945 domain-containing protein	-
HPE49_RS17705	3675371..3675844	+	474	WP_001296921.1	DNA repair protein RadC	-
HPE49_RS17710	3675844..3676179	+	336	WP_001296928.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPE49_RS17715	3676210..3676551	+	342	WP_001683515.1	TA system toxin CbtA family protein	Toxin
HPE49_RS17720	3676666..3677499	+	834	WP_032218973.1	DUF4942 domain-containing protein	-
HPE49_RS17725	3677576..3677824	+	249	WP_032218975.1	ribbon-helix-helix domain-containing protein	-
HPE49_RS17730	3677828..3678103	+	276	WP_032218977.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPE49_RS17735	3678217..3679014	+	798	WP_032218979.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	3664519..3690179	25660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13034.05 Da
• Isoelectric Point: 9.5454

>T159492 WP_001683515.1 NZ_CP054363:3676210-3676551 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T159492 NZ_CP070063:c3301921-3301670 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12565.57 Da
• Isoelectric Point: 7.7245

>AT159492 WP_001296928.1 NZ_CP054363:3675844-3676179 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKHLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT159492 NZ_CP070063:c3301673-3301419 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A4T3Q6D2