Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2571530..2572161 Replicon chromosome
Accession NZ_CP054363
Organism Escherichia coli strain SCU-171

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag HPE49_RS12460 Protein ID WP_001260301.1
Coordinates 2571886..2572161 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID L4IZA1
Locus tag HPE49_RS12455 Protein ID WP_000593557.1
Coordinates 2571530..2571889 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE49_RS12430 2567662..2568606 + 945 WP_000947063.1 nickel ABC transporter permease subunit NikB -
HPE49_RS12435 2568603..2569436 + 834 WP_042105887.1 nickel ABC transporter permease subunit NikC -
HPE49_RS12440 2569436..2570200 + 765 WP_001136206.1 nickel import ATP-binding protein NikD -
HPE49_RS12445 2570197..2571003 + 807 WP_000173650.1 nickel import ATP-binding protein NikE -
HPE49_RS12450 2571009..2571410 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HPE49_RS12455 2571530..2571889 - 360 WP_000593557.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HPE49_RS12460 2571886..2572161 - 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
HPE49_RS12465 2572234..2573358 - 1125 WP_001318088.1 ABC transporter permease -
HPE49_RS12470 2573358..2576093 - 2736 WP_052920229.1 ribosome-associated ATPase/putative transporter RbbA -
HPE49_RS12475 2576090..2577157 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T159486 WP_001260301.1 NZ_CP054363:c2572161-2571886 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T159486 NZ_CP070063:c2507818-2507711 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13389.28 Da        Isoelectric Point: 5.1987

>AT159486 WP_000593557.1 NZ_CP054363:c2571889-2571530 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT159486 NZ_CP070063:2507874-2507935 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB L4IZA1

References