Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1748614..1749268 Replicon chromosome
Accession NZ_CP054363
Organism Escherichia coli strain SCU-171

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HPE49_RS08385 Protein ID WP_000244781.1
Coordinates 1748614..1749021 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HPE49_RS08390 Protein ID WP_000354046.1
Coordinates 1749002..1749268 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE49_RS08365 1744571..1746304 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
HPE49_RS08370 1746310..1747020 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HPE49_RS08375 1747045..1747941 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HPE49_RS08380 1748053..1748574 + 522 WP_001055874.1 flavodoxin FldB -
HPE49_RS08385 1748614..1749021 - 408 WP_000244781.1 protein YgfX Toxin
HPE49_RS08390 1749002..1749268 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HPE49_RS08395 1749521..1750501 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
HPE49_RS08400 1750578..1751237 - 660 WP_000250269.1 hemolysin III family protein -
HPE49_RS08405 1751401..1751712 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
HPE49_RS08410 1751757..1753190 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T159482 WP_000244781.1 NZ_CP054363:c1749021-1748614 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159482 NZ_CP070063:1259013-1259327 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159482 WP_000354046.1 NZ_CP054363:c1749268-1749002 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159482 NZ_CP070063:1258777-1259010 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References