Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1286563..1287188 Replicon chromosome
Accession NZ_CP054363
Organism Escherichia coli strain SCU-171

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HPE49_RS06290 Protein ID WP_000911329.1
Coordinates 1286563..1286961 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HPE49_RS06295 Protein ID WP_000450524.1
Coordinates 1286961..1287188 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE49_RS06270 1282470..1282670 + 201 WP_000383836.1 YpfN family protein -
HPE49_RS06275 1282751..1283449 - 699 WP_000679819.1 esterase -
HPE49_RS06280 1283523..1285538 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
HPE49_RS06285 1285553..1286416 - 864 WP_042104743.1 neutral zinc metallopeptidase -
HPE49_RS06290 1286563..1286961 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
HPE49_RS06295 1286961..1287188 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HPE49_RS06300 1287344..1288057 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HPE49_RS06305 1288270..1289304 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HPE49_RS06310 1289321..1290199 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HPE49_RS06315 1290345..1290917 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HPE49_RS06320 1290917..1291387 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T159481 WP_000911329.1 NZ_CP054363:c1286961-1286563 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T159481 NZ_CP070063:c1222066-1221914 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT159481 WP_000450524.1 NZ_CP054363:c1287188-1286961 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT159481 NZ_CP070063:1222115-1222172 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References