Detailed information of TA system TA159479

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 778674..779505
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HPE49_RS03905
• Protein ID: WP_000854814.1
• Coordinates: 779131..779505 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: HPE49_RS03900
• Protein ID: WP_001285585.1
• Coordinates: 778674..779042 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS03865	774672..775502	+	831	Protein_758	hypothetical protein	-
HPE49_RS03870	775578..776033	+	456	WP_000581504.1	hypothetical protein	-
HPE49_RS03875	776112..776345	+	234	WP_001119729.1	DUF905 family protein	-
HPE49_RS03880	776445..777263	+	819	WP_001234629.1	DUF945 domain-containing protein	-
HPE49_RS03885	777345..777824	+	480	WP_000860076.1	antirestriction protein	-
HPE49_RS03890	777840..778316	+	477	WP_001186773.1	RadC family protein	-
HPE49_RS03895	778379..778600	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HPE49_RS03900	778674..779042	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HPE49_RS03905	779131..779505	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HPE49_RS03910	779502..779696	+	195	WP_000988600.1	hypothetical protein	-
HPE49_RS03915	779709..779822	+	114	WP_032154230.1	DUF957 domain-containing protein	-
HPE49_RS03920	780111..780239	-	129	Protein_769	transposase domain-containing protein	-
HPE49_RS03925	780311..780493	+	183	WP_001016348.1	hypothetical protein	-
HPE49_RS03930	780594..780923	-	330	WP_000450409.1	DUF496 family protein	-
HPE49_RS03935	781095..782153	-	1059	WP_001200891.1	FUSC family protein	-
HPE49_RS03940	782351..782824	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
HPE49_RS03945	782943..784109	-	1167	WP_042105928.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T159479 WP_000854814.1 NZ_CP054363:779131-779505 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T159479 NZ_CP070063:927212-927385 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT159479 WP_001285585.1 NZ_CP054363:778674-779042 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT159479 NZ_CP070063:926888-927184 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60