Detailed information of TA system TA159470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 355428..355907
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A8S7NJ77
• Locus tag: HPE49_RS01730
• Protein ID: WP_042105936.1
• Coordinates: 355620..355907 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A193LPI7
• Locus tag: HPE49_RS01725
• Protein ID: WP_042105938.1
• Coordinates: 355428..355619 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS01670	350428..350772	-	345	WP_174144370.1	ead/Ea22-like family protein	-
HPE49_RS01675	350759..351064	-	306	WP_077886833.1	DUF4406 domain-containing protein	-
HPE49_RS01680	351061..351336	-	276	WP_077886881.1	DNA-binding protein	-
HPE49_RS01685	351369..352085	-	717	WP_174144372.1	DUF1627 domain-containing protein	-
HPE49_RS01690	352114..352854	-	741	WP_174144373.1	ATP-binding protein	-
HPE49_RS01695	352861..353190	-	330	Protein_335	hypothetical protein	-
HPE49_RS01700	353245..353760	-	516	Protein_336	helix-turn-helix domain-containing protein	-
HPE49_RS01705	353783..354208	-	426	WP_042105942.1	Rha family transcriptional regulator	-
HPE49_RS01710	354205..354492	-	288	WP_042105941.1	helix-turn-helix domain-containing protein	-
HPE49_RS01715	354595..354966	+	372	WP_148935084.1	hypothetical protein	-
HPE49_RS01720	354997..355392	+	396	WP_042105939.1	helix-turn-helix domain-containing protein	-
HPE49_RS01725	355428..355619	+	192	WP_042105938.1	hypothetical protein	Antitoxin
HPE49_RS01730	355620..355907	+	288	WP_042105936.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPE49_RS01735	356171..356428	+	258	WP_174144317.1	hypothetical protein	-
HPE49_RS01740	356382..356639	-	258	WP_042105945.1	hypothetical protein	-
HPE49_RS01745	357139..357327	+	189	WP_174144375.1	cell division inhibitor	-
HPE49_RS01750	357492..359984	+	2493	WP_174144377.1	exonuclease	-
HPE49_RS01755	360055..360306	+	252	WP_000005551.1	excisionase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	295052..373376	78324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10968.73 Da
• Isoelectric Point: 6.2118

>T159470 WP_042105936.1 NZ_CP054363:355620-355907 [Escherichia coli]
MLPVLWLESADADLDDITSYIARFDIDAAERLWKRLRTCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T159470 NZ_CP070048:4822335-4822676 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7364.29 Da
• Isoelectric Point: 5.8256

>AT159470 WP_042105938.1 NZ_CP054363:355428-355619 [Escherichia coli]
MNTALSPMVSEFETIEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFANMRKERSKQ

Nucleotide

• Length: 192 bp

>AT159470 NZ_CP070048:4822686-4822985 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A193LPI7