Detailed information of TA system TA159468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 182643..183279
• Replicon: chromosome
• Accession: NZ_CP054363
• Organism: Escherichia coli strain SCU-171

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: HPE49_RS00850
• Protein ID: WP_000813796.1
• Coordinates: 182643..182819 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HPE49_RS00855
• Protein ID: WP_076838470.1
• Coordinates: 182863..183279 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE49_RS00830	178265..179476	-	1212	WP_071593090.1	BenE family transporter YdcO	-
HPE49_RS00835	179529..180065	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
HPE49_RS00840	180138..182099	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HPE49_RS00845	182191..182421	-	231	WP_000494243.1	YncJ family protein	-
HPE49_RS00850	182643..182819	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HPE49_RS00855	182863..183279	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HPE49_RS00860	183358..184764	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
HPE49_RS00865	185009..186154	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
HPE49_RS00870	186172..187185	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
HPE49_RS00875	187186..188127	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T159468 WP_000813796.1 NZ_CP054363:182643-182819 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T159468 NZ_CP070048:c4432815-4432474 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT159468 WP_076838470.1 NZ_CP054363:182863-183279 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT159468 NZ_CP070048:4432810-4432886 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3