Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4832933..4833564 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name hicA Uniprot ID D8A4M2
Locus tag HPE50_RS23995 Protein ID WP_001259770.1
Coordinates 4833289..4833564 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HPE50_RS23990 Protein ID WP_000593555.1
Coordinates 4832933..4833292 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS23960 4828048..4828521 + 474 WP_001572025.1 PTS sugar transporter subunit IIA -
HPE50_RS23965 4828518..4828799 + 282 WP_000084021.1 PTS sugar transporter subunit IIB -
HPE50_RS23970 4828873..4830231 + 1359 WP_001329802.1 PTS galactitol transporter subunit IIC -
HPE50_RS23975 4830224..4831732 + 1509 WP_001329801.1 carbohydrate kinase -
HPE50_RS23980 4831722..4831991 + 270 WP_000949581.1 HPr family phosphocarrier protein -
HPE50_RS23985 4832023..4832883 + 861 WP_001131792.1 class II aldolase -
HPE50_RS23990 4832933..4833292 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HPE50_RS23995 4833289..4833564 - 276 WP_001259770.1 type II toxin-antitoxin system HicA family toxin Toxin
HPE50_RS24000 4833637..4834761 - 1125 WP_001314210.1 ABC transporter permease -
HPE50_RS24005 4834761..4837496 - 2736 WP_001572026.1 ribosome-associated ATPase/putative transporter RbbA -
HPE50_RS24010 4837493..4838560 - 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10170.90 Da        Isoelectric Point: 10.6128

>T159459 WP_001259770.1 NZ_CP054353:c4833564-4833289 [Escherichia coli]
MRTLVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T159459 NZ_CP070048:c1711149-1710898 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT159459 WP_000593555.1 NZ_CP054353:c4833292-4832933 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT159459 NZ_CP070048:c1710901-1710647 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References