Detailed information of TA system TA159457

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4289003..4289837
• Replicon: chromosome
• Accession: NZ_CP054353
• Organism: Escherichia coli strain SCU-172

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: HPE50_RS21265
• Protein ID: WP_000854689.1
• Coordinates: 4289460..4289837 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: HPE50_RS21260
• Protein ID: WP_001546108.1
• Coordinates: 4289003..4289383 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE50_RS21215	4285012..4285467	+	456	WP_000581502.1	hypothetical protein	-
HPE50_RS21220	4285568..4285777	+	210	Protein_4134	DUF905 family protein	-
HPE50_RS21225	4285878..4286699	+	822	WP_174183274.1	DUF945 domain-containing protein	-
HPE50_RS21230	4286699..4286946	+	248	Protein_4136	antirestriction protein	-
HPE50_RS21235	4287041..4287514	+	474	WP_001571910.1	antirestriction protein	-
HPE50_RS21240	4287530..4288006	+	477	WP_001186774.1	RadC family protein	-
HPE50_RS21245	4288069..4288290	+	222	WP_000692315.1	DUF987 domain-containing protein	-
HPE50_RS21250	4288309..4288452	+	144	WP_001571912.1	hypothetical protein	-
HPE50_RS21255	4288510..4288953	+	444	WP_001571913.1	hypothetical protein	-
HPE50_RS21260	4289003..4289383	+	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HPE50_RS21265	4289460..4289837	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
HPE50_RS21270	4289834..4290322	+	489	WP_001530850.1	hypothetical protein	-
HPE50_RS21275	4290339..4290536	+	198	WP_000839250.1	DUF957 domain-containing protein	-
HPE50_RS21280	4290621..4291466	+	846	WP_001530851.1	DUF4942 domain-containing protein	-
HPE50_RS21285	4291535..4291930	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HPE50_RS21290	4291923..4292856	+	934	Protein_4148	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HPE50_RS21295	4293288..4293443	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4293288..4293443	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T159457 WP_000854689.1 NZ_CP054353:4289460-4289837 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T159457 NZ_CP070048:1699005-1699379 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT159457 WP_001546108.1 NZ_CP054353:4289003-4289383 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT159457 NZ_CP070048:1698548-1698916 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8