Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4119592..4120246 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HPE50_RS20350 Protein ID WP_000244781.1
Coordinates 4119592..4119999 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HPE50_RS20355 Protein ID WP_000354046.1
Coordinates 4119980..4120246 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS20330 4115549..4117282 - 1734 WP_000813179.1 single-stranded-DNA-specific exonuclease RecJ -
HPE50_RS20335 4117288..4117998 - 711 WP_000715219.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HPE50_RS20340 4118023..4118919 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HPE50_RS20345 4119031..4119552 + 522 WP_001571858.1 flavodoxin FldB -
HPE50_RS20350 4119592..4119999 - 408 WP_000244781.1 protein YgfX Toxin
HPE50_RS20355 4119980..4120246 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HPE50_RS20360 4120488..4121468 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HPE50_RS20365 4121545..4122204 - 660 WP_000250274.1 hemolysin III family protein -
HPE50_RS20370 4122368..4122679 - 312 WP_001182939.1 N(4)-acetylcytidine aminohydrolase -
HPE50_RS20375 4122724..4124157 + 1434 WP_001571859.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T159456 WP_000244781.1 NZ_CP054353:c4119999-4119592 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159456 NZ_CP070048:c1480972-1480869 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159456 WP_000354046.1 NZ_CP054353:c4120246-4119980 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159456 NZ_CP070048:1480831-1481102 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References