Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2536666..2537192 Replicon chromosome
Accession NZ_CP054353
Organism Escherichia coli strain SCU-172

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HPE50_RS12665 Protein ID WP_000323025.1
Coordinates 2536666..2536953 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HPE50_RS12670 Protein ID WP_000534858.1
Coordinates 2536953..2537192 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE50_RS12620 2531690..2531905 - 216 WP_000839590.1 phage lysis protein EssD -
HPE50_RS12625 2532659..2532874 - 216 WP_000066484.1 cold shock-like protein CspB -
HPE50_RS12630 2533175..2533387 + 213 WP_000087756.1 cold shock-like protein CspF -
HPE50_RS12635 2533442..2533531 + 90 WP_120795389.1 hypothetical protein -
HPE50_RS12640 2533809..2534561 - 753 WP_001571462.1 antitermination protein -
HPE50_RS12645 2534575..2535624 - 1050 WP_001265198.1 DUF968 domain-containing protein -
HPE50_RS12650 2535626..2535904 - 279 WP_032147666.1 hypothetical protein -
HPE50_RS12655 2535971..2536222 - 252 WP_000980988.1 hypothetical protein -
HPE50_RS12660 2536439..2536594 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HPE50_RS12665 2536666..2536953 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HPE50_RS12670 2536953..2537192 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HPE50_RS12675 2537217..2537522 + 306 WP_001326990.1 hypothetical protein -
HPE50_RS12680 2537725..2538057 + 333 WP_001301033.1 protein FlxA -
HPE50_RS12685 2538494..2539807 - 1314 WP_001571463.1 ISNCY family transposase -
HPE50_RS12690 2539985..2540167 + 183 WP_000955178.1 hypothetical protein -
HPE50_RS12695 2541474..2541830 - 357 WP_001310834.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2505304..2563182 57878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T159452 WP_000323025.1 NZ_CP054353:c2536953-2536666 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T159452 NZ_CP070048:c796212-796105 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT159452 WP_000534858.1 NZ_CP054353:c2537192-2536953 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT159452 NZ_CP070048:796265-796326 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References